1946
– Kentucky Chamber of Commerce incorporated. Earl Muir, volunteer president; Louis Quinn,  secretary.

1947
– Chamber began building coalitions with other associations by appointing "state councilors" to act as liaisons to trade and civic organizations.
– Chamber formed its first committee with taxation as its focus.

1949
– George Mascott hired as the first full-time executive secretary of the Chamber.
– Community Development Program established. It evolved into the All-Kentucky Cities, Certified Cities and today’s Certified Communities Partnership Program. First test site in London and Laurel County.

1953
– Harper Gatton named executive secretary.
– Main Street Modernization Program developed to help Kentucky merchants increase sales and make communities more attractive to industry and tourists.

1954
–  Kentucky Chamber of Commerce Business and Industry Day planned in conjunction with the State Fair Committee.

1955
–  In partnership with the University of Kentucky, the Chamber launched a program to promote agricultural income in Kentucky. Mayfield and Somerset were chosen as the first sites for workshops. 

1957
– The Chamber polled members on top concerns. The top five were industrial development, education, the business climate, highways and community development.

1958
–  Kentucky Chamber awarded U.S. Chamber citation for all-around achievement, citing work in "business development, community betterment, legislative action, economic understanding and organizational improvement."

1959
–  Chamber continued business advocacy role by sending a telegram to Gov. Chandler urging him to protect the unemployment trust fund and use its monies solely for their intended purpose.

1961
–  Jim C. Zimmerman named executive vice president.

1963
–  Chamber created college-business symposium to create opportunity for direct communication between business executives and students.

1964
–  Chamber launched program to study the progress of education and encourage business support in raising state education levels.

1965
–  Chamber and Kentucky Department of Commerce sponsored luncheon during Industry Appreciation Week to recognize 76 new plants and their expansions for bringing in 7,519 new jobs and $83,858,840.

1966
–  John M. Lewis named executive vice president.
–  Chamber established an education division and hired its director.

1970
–  Chamber member survey revealed new top priorities: (1) education (2) civil disorder management and (3) pollution control.
–  Priorities demonstrated need for increased citizen action. Six councils set up to facilitate the following: community development, education and public affairs, economic development, taxation and government affairs, labor/ management relations and travel development.

1972
–  Chamber established Kentucky Night in Washington as an opportunity for members to meet with congressional delegates.
–  Philip Thompson named Chamber president (title changed from executive vice president). 

1973
–  Chamber moved from Louisville to Frankfort as organization mission shifted its primary focus from industrial recruiting to lobbying state government.

1974
–  "Destination: Kentucky" established as an arm of the Chamber, enabling Kentuckians to call a toll-free reservation line and make reservations at state parks.
–  Chamber state fair luncheons provided a forum for candidates running for political office. The Chamber also presented awards to high school students who demonstrated leadership and academic excellence.

1977
–  Chamber created an economic education and research foundation to deal with economic illiteracy.

1981
–  An independent study of legislators named the Chamber the "most powerful and influential of all business organizations or companies lobbying the General Assembly."

1983
–  Kentuckians for Excellence in Education founded; instigated KERA, the 1990 Kentucky Education Reform Act.

1984
–  Leadership Kentucky founded to inspire and educate Kentucky’s business leaders.
–  Jim Wiseman named Chamber president.

1988
–  Chamber established arrangement with Blue Cross and Blue Shield of Kentucky to provide Chamber customers with a competitively priced group medical benefit program called ChamberAdvantage.

1989
–  Project 21 founded as a long-term strategic planning effort.

1992
–  Ken Oilschlager named Chamber president.

1993
–  Chamber founded KARE, the Kentucky Association of Responsible Employers, as a self-insured group fund designed to provide a stable alternative for members’ workers’ compensation insurance.
–  Chamber moved to current location adjacent to I-64 in Frankfort.

1994
–  Chamber stopped a proposed eyewash safety standard, which would have required tempered water and weekly testing of eyewash and safety shower units, costing Kentucky companies millions to comply.
–  Chamber opposed House Bill 455 because it would have tripled the ad valorem tax manufacturers pay on machinery used in the manufacturing process.

1995
–  Kentucky Chamber created the Kentucky Chamber Foundation exclusively for charitable and educational purposes.
–  Chamber Services Division formed as a wholly owned subsidiary to develop and market insurance and value-added products to Chamber customers.
–  Chamber helped form EPIC, the Economic Progress Initiative Council, a coalition of business and trade associations designed to address specific business concerns before the public and the legislature.

1996
–  Chamber helped pass House Bill 1, which reformed Kentucky’s workers’ compensation system and is considered among the Chamber’s most significant victories.

1997
–  Chamber directors established four strategic public policy priorities for the Kentucky Chamber Foundation’s program of work.

2002
–  Chamber establishes the Kentucky Employers Health Benefits Coalition to address the dramatically rising cost of health care.

2003
–  Chamber board of directors adopts its New Horizons program, establishing new membership levels: Advocates, Chair's Council and Presidential Advisors.